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Microbiology general microbiology 5a736b35

All of the following are used in Gram's staining except?

A
Iodine
B
Alcohol
C
Congo red
D
Crystal violet
High-Yield Explanation
GRAM STAINING 1)Primary staining with a pararosaniline dye such as crystal violet, methyl violet, or gentian violet 2)Application of a dilute solution of iodine 3)Decolourisation with an inorganic solvent such as ethanol, acetone, or aniline 4)Counterstaining with a dye of contrasting color such as carbon fuchsin, safranine or neutral red Congo Red: It is an organic dye, an azo dye mainly used in the diagnosis of amyloidosis REF:Ananthanarayan & Panicker's Textbook of Microbiology 9th Edition pg no: 13

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