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Pathology General pathology 5085b502

In-situ DNA nick end labeling can quantitate -

A
Fraction of cells in apoptotic pathways
B
Fraction of cells in S phase
C
p53 gene produce
D
bcr/abl gene
High-Yield Explanation
In Situ Nick-End Labeling An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION. Ref Robbins 9/e p218

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