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Biochemistry Carbohydrates 4b7b0fff

The reaction catalyzed by phosphofructokinase:

A
Is activated by high concentrations of ATP and citrate
B
Uses fructose-l-phosphate as substrate
C
Is the rate-limiting reaction of glycolytic Pathway?
D
Is inhibited by fructose 2, 6-bisphosphate
High-Yield Explanation
Ans. C. Is the rate-limiting reaction of glycolytic PathwayPhosphofructokinase is the key enzyme of glycolysis. It phosphorylates Fructose-6- phosphate to form Fructose 1,6- bisphosphate. It is inhibited by ATP and Citrate ions and allosterically modified by AMP. PFK has two activities. PFK1 forms Fructose 1,6- bisphosphate and PFK2 forms Fructose 2,6-bisphosphate. The former reaction is irreversible and the latter reversible. Fructose 2,6-bisphosphate depicts the energy status of the body.

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