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Microbiology All India exam 41e27169

all the following statements about hybridoma technology are true except :

A
specific antibody producing cells are integrated with myeloma cell
B
myeloma cells in salvage pathway grows well in HAT medium
C
aminopterin, a folate antagonist inhibits denovo pathway
D
HGP ase and thymidylate sytnthtase are required for salvage pathway
High-Yield Explanation
myeloma cells lack HGPase enzyme hence they cannot grow well in HAT medium APPLICATION OF HYBRIDOMA TECHNOLOGY Serological, * Identification of ABO blood group Diagnosis: * Detection of pregnancy by assaying of hormones with monoclonal. * Separation of one substance from a mixture of very similar molecules. Immunopurification: * Purification of individual interferon using monoclonal. * Inactivation of T-Iymphocytes responsible for rejection of organ transplants. Therapy: * Removal of tumor cell from bone marrow. * Treatment of acute renal failure. * Treatment malignant leukemic cells, B cell lymphomas, and a variety of allograft rejections after transplantation. ref : ananthanaryana 9th ed

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