Biochemistry General 3e1d16fb Which of the following is true for phosphofructokinase A It uses fructose 1, 6 bisphosphate as substrate B It generates ATP C It catalyses an irreversible reaction in glycolysis D It is activated by increased levels of ATP and citrate High-Yield Explanation Phosphofructokinase catalyses the first committed step of glycolysis. It is the rate limiting enzyme of glycolysis. The reaction is irreversible.