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Biochemistry Miscellaneous (Bio-Chemistry) 3799807c

Which of the following is true regarding Fluorescence?

A
Spontaneous illumination in dark
B
Release of longer wavelength light on absorbing light of shorter wavelength
C
Release of shorter wavelength light on absorbing light of longer wavelength
D
Continuous emission of lights of different wavelength
High-Yield Explanation
Ans. b. Release of longer wavelength light on absorbing light of shorter wavelength (Ref: Tietz' Fundamentals of Clinical Chemistry 5/e p75; Lehninger 4/447; Harper 29/e p311)In Fluorescence, there is release of longer wavelength of light on absorbing light of shorter wavelength is seen.FluorescenceIt is the emission of light by a substance that has absorbed light or other electromagnetic radiationQ.It is a form of luminescenceQIn most cases, the emitted lights have a longer wavelength and lower energy than the absorbed radiationQ.

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