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Microbiology Mycology 37732a33

Test used to diagnosis Pneumocystis Jirovecci -

A
Methenamine silver
B
PCR
C
Immunofluorescence
D
All of the above
High-Yield Explanation
Ans. is 'd' i.e., All of the above Diagnosis of Pneumocystis Tirovecci* Best specimen is bronchoscopy with BAL (bronchoalveolar lavage) fluid. Sputum can also be used.* Following are methods for diagnosisi) Histopathologic staining for cysts and other stages: It is most commonly used method for diagnosis. Methenamine silver, toluidine blue and crystal violet stain the cyst wall. Wright Geimsa stains all development stages.ii) Immunofluorescence with monoclonal antibodies.iii) PCR: It is most sensitive.

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