Which of the following is non cultiviable invivo

i.e. (Hepatitis C- Virus) : (432-35-Ananthanarayan 8th )Explanation : Some viruses are not culturable in vitro due to ack of susceptible cell lines, e.g. certain blood- borne (e.g. Hepatitis C virus, HCV) and water -borne (e.g. Norwalk virus) viruses.MYCOPLASMAMycoplasma - smallest free living organism known are prokaryotes that are bounded only by a plasma membrane* Their lack of a cell wall is associated with cellular pleomorphism and resistance to cell wall active antimicrobial agents such as penicilline and cephalosporins (beta lactams, vancomycin, Bacitracin)* The 'organism ' small genome limits biosynthesis and explains the difficulties encountered with in vitro cultivation (1068-H17th)* Myeo, pneumoniae can be grown on artificial media, but the process is exacting, requires special media and takes more than 2 weeks. Thus mycoplasma cultures do not provide any timely information* Mycoplasma are bounded by a triple layered unit membrane that contains s sterol therefore when mycoplasma are grown in culture media, the media required for the growth of the organsm should be rich in sterol (This is provided by adding 20% horse or human serum)