Rate limiting step in Gluconeogenesis is catalyzed by

Key Gluconeogenic Enzymes 1.Pyruvate carboxylase. 2. Phosphoenolpyruvate carboxykinase 3.Fructose-1-6-bisphosphatase 4.Glucose-6-phosphatase in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase (Fig. 9.24). It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, page no: 117