Which of the following is the primary cell line -

Human diploid fibroblasts (HDF) cell line is used for cultivation of coxsackie A7,9,16; CMV; Echovirus; enteroviruses; HSV-1,2; Poliovirus: parainfluenza virus, rhinovirus and VZV. Cultivation of viruses ‘As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium.' Three methods are employed for cultivation of viruses : 1. Animal Inoculation  Earliest method  Mice are most widely employed animals  Other animals used are guinea pigs, rabbits and ferrets. 2. Embryonated eggs  Sites of inoculation are: a) Chorioallantoic Membrane ------- visible pocks are produced → HSV, Vaccinia, Smallpox b) Amniotic Sac c) Allantoic cavity →  Influenza       Some paramyxoviruses d) Yolk Sac → Some viruses      Chlamydia      Rickettsia 3.Tissue culture  Most important method for virus isolation.  Three types of tissue culture are available I. Organ Culture II Explant Culture III. Cell Culture - (routinely employed) Cell culture may be 4. Primary cell culture Rhesus monkey kidney, human amnion, chick embryo fibroblast, human embryonic kidney. 5. Diploid cell strains:- Human diploid fibroblast HL-8, WI-38. 6. Continuous cell line:- Hela, HEP-2, McCoy, Vero.