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Microbiology General 29312fa4

The chamber condition required for autoclaving with respect to the temperature-time is which of the following?

A
121degC temperature for 20 min
B
121degC temperature for 15 min
C
100degC temperature for 60 min
D
100degC temperature for 90 min
High-Yield Explanation
Moist heat sterilization, usually autoclaving, is the most frequently used method of sterilization. Bacterial spores which are resistant to boiling are sterilized using autoclaving. Autoclaving is done in a chamber in which steam, at a pressure of 15 lb/in2, reaches a temperature of 121degC and is held at that temperature for 15 to 20 minutes. Sterilization by dry heat requires temperatures in the range of 180degC for 2 hours. It is used primarily for glassware. Pasteurization involves heating the milk to 62degC for 30 minutes followed by rapid cooling. It is sufficient to kill the vegetative cells of the milk-borne pathogens (e.g., Mycobacterium bovis, Salmonella, Streptococcus, Listeria, and Brucella). Ref: Review of Medical Microbiology and Immunology, 12e, Chapter 13

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