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Microbiology general microbiology 2703ec16

One of the following staining methods is an example of Negative Staining -

A
Gram's staining
B
Fontana's staining
C
India Ink Preparation
D
Ziehl-Neelsen staining
High-Yield Explanation
Negative staining India ink or Nigrosin are emulsified with the simple or organism to provide a uniformly colored background against which the unstained bacteria stand out in contrast. This is paicularly useful in the demonstration of bacteria capsules which do not take simple stains. Very slender bacteria such as spirochetes that are not demonstrable by simple staining methods can be viewed by negative staining. Ref :Ananthanarayan & paniker's Textbook of Microbiology9th edition pg no 12

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