According to WHO criteria, the minimum normal sperm count is :

Ans. is b i.e. 20 million/ml Volume Sperm concentration Sperm motility Normal morphology Immunological or mixed agglutination reaction test White blood cells 2 ml > 20 million / ml > 50% progressive or > 25% rapidly progressive > 50% normal forms < 10% coated < 1 million per ml Normal seminal fluid analysis : 20 million / ml > 50% progressive or > 25% rapidly progressive > 50% normal forms < 10% coated < 1 million per ml " align="left" height="100" width="265">Other impoant values in semen Analysis : pH = > 7.2 Round cells (including WBC + epithelial cells + immature cells) = < 5 million/ml. Sperm agglutination = <2 Basic semen Analysis includes only 4 measures : 1. Semen volumedeg Sperm concentrationdeg Motilitydeg Morpohologydeg The specimen for semen analysis should be collected after 2 - 3 days of abstinence. The specimen should be obtained by masturbation. The specimen should be reach the laboratory within an hour of ejaculation. Extra Edge : Besides WHO criteria for evaluating sperm morphology, another 'strict criteria' by Kruger and Tygerberg is gaining impoance. Strict sperm morphology represents the best current predictor of sperm function (the capacity to feilize a mature oocyte) and has emerged as the most discriminating factor among infeile and feile men. If > 14% sperms are of normal morphology by the strict criteria then only the semen considered as normal. Teratospermia is < 4% normal sperms by the strict criteria and is an indicator of poor prognosis in assisted feilization by ICSI in IVF cycles. When no motile sperms are observed a sperm bility test differentiates ble non motile sperms from dead sperms. Round cells in semen analysis includes epithelial cells. prostate cells. immature sperms (spermatogonia, round spermatids, spermatocytes) and leucocytes. If total round cells is > 5 million/ml it is abnormal. True leukocytospermia means > 1 million leukocytes/ml and requires semen culture for mycoplasma hominis, ureoplasma ureolyticum and Chlamydia. Lymphocytes can be distinguished from other cells by immunoperoxide staining "Endtz Test".