Which of the following statement is true regarding Acanthamoeba keratitis?

For the isolation of causative agent, corneal scraping should be cultured on non-nutrient agar (not nutrient agar). 'Culture on non-nutrient agar (E. coli enriched) may show trophozoites within 48 hrs : which gradually turn into yeasts. - khurana Normal habitat is soil, water and dust but it is an amoeba (not a helminth) Keratitis due to Acanthamoeba may be seen both in immunocompetent and immunocompromised hosts. In contrast to many another pathogenic amoeba, this organism is not naturally parasitic and does not require a host.