Which of these are seen on Romanowsky stain -a) Reticulocytesb) Basophilic stipplingc) Heinz bodiesd) Howell-Jolly bodiese) Cabot ring

Romanowsky dyes are used for staining blood films. They are made up of combination of acid and basic dyes. The nucleus and neutrophilic granules are basophilic and stains blue. Hemoglobin is acidophilic and stains red. Various modifications available are Leishman’s stain, Wright’s stain, Giemsa and Jenner’s stain. Basophilic stippling, Howell-Jolly body and Cabot rings are seen by Romanowsky stain. Basophilic stippling: These are small blue or black granules in red cells seen in megaloblastic anemia, heavy metal poisonings, etc. Howell-Jolly Body: These are remnants of the nucleus seen as small, round dark blue particles near the periphery of the cells; found in postsplenectomy, asplenia and severe hemolytic anemia. Cabot ring: These are pale staining nuclear remnants in the form of rings or figure of eight seen in hemolytic anemia, megaloblastic anemia, leukemia and after splenectomy. These are arginine rich and acidophilic. Heinz bodies are denatured hemoglobin which does not stained with Romanowsky stain. It is demonstrated by supravital stains such as crystal violets. Reticulocytes also require Supravital staining.