Conclusive test for semen

The Acid Phosphatase Test: The prostatic secretion element of seminal fluid contains 500 to 1000 times greater acid phosphatase than any other body fluid. Undiluted semen has an acid phosphatase activity of 340 to 360 Bodanskyunits or 2500 to 3500 Angstrom units per ml. The concentration of acid phosphatase gradually falls with time in vaginal secretions, and positive reactions are found for periods of thiysix hours, with gradual disappearance in 72 hrs. Five to ten ml of normal saline solution is placed in the vagina with a syringe. The fluid is then removed and placed in a sealed tube and refrigerated for enzyme examination. Concentration in excess of hundred Bodansky units with or without motile sperms indicate that ejaculation occurred within twelve hours of examination. Dried seminal stains which have not undergone putrefaction retain acid phosphatase activity for weeks or months, although enzymatic activity decreases slowly with time. This test is conclusive in the absence of demonstrable sperms or in aspermia. Ref:- k s narayan reddy; pg num:-434,435