Inspissation is -

Ans. is 'c' i.e., Heating at 80degC for 30 minutes Inspissation* It is heat exposure method that is employed with high protein material, like egg containing media, that cannot withstand the high temperature used in autoclaving.* The medium is exposed to 80degC for 30 minutes for three successive days in inspissator.* It can be used for LJ medium and loeffler serum slop.