Substrate affinity to enzyme in Competitive inhibition

Significance of Km (Michaelis Constant): Km is substrate concentration at 1/2 Vmax Constant for an enzyme substrate pair It is called signature of the enzyme Higher the Km, lower is the affinity of the enzyme towards the substrate Lower the Km, higher is the affinity of the enzyme towards the substrate. Features of competitive inhibition: Km increases, hence the affinity is lowered Vmax remains the same. Features of noncompetitive inhibition: Km remains the same Vmax decreases.