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Biochemistry General 078d4e1b

The following methods can be used to detect the point mutation in the beta globin gene that causes sickle cell anemia, EXCEPT:

A
Polymerase chain reaction with allele-specific oligonucleotide hybridization
B
Southern blot analysis
C
DNA sequencing
D
Nohern blot analysis
High-Yield Explanation
Point mutation involves the replacement of a single base nucleotide with another nucleotide of the gene. Point mutations in the beta globin gene can be identified by analysing the DNA. Nohern blot is a technique used in studying the RNA and hence the answer of exclusion. Ref: Introduction to Biotechnology By Thieman William, 2nd Edition, Pages 71, 78, 81, 82

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