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Biochemistry Metabolism of nucleic acids 05fe8f4a

For RNA, which blotting technique is used

A
Western blot
B
Nohern blot
C
Southern blot
D
None
High-Yield Explanation
Nohern Blotting for identifying RNA : The Nohern blot is used to demonstrate specific RNA. The total RNA is isolated from the cell, electrophoresed and then blotted on to a membrane. This is then probed with radioactive cDNA .There will be RNA - DNA hybridization. This is used to detect the gene expression in a tissue. Ref :DM.VASUDEVAN.TEXTBOOK ; SEVENTH EDITION ; PAGE NO : 634.

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